![]() This study is promising for the future, as it will make it possible to create a MALDI-TOF MS database from a wide range of ticks which have been stored for a long time in alcohol or which are dry stored in laboratories and museums around the world.Īlcohol preservation Entomology MALDI-TOF MS Tick.Ĭopyright © 2022 Elsevier B.V. The analysis of the results showed that among the four protocols tested, two protocols named ReDO (Rehydration and incubation of the legs in 40 μl of HPLC water for 12 h in a dry bath at 37°) and PreDO (Drying of the legs for 12 h in a dry bath at 37 ☌ followed by rehydration and incubation in 40 μl of HPLC water for 12 h.) seem to be more appropriate for the MALDI-TOF MS identification of ticks from old collections preserved in alcohol or dry. In this study, a standard protocol was established for the identification of tick collections which had been stored for more than ten years in alcohol. However, previous studies reported that preservation of arthropods in alcohol modified the MS spectra obtained from specimens of the same species freshly collected or frozenly stored. MALDI-TOF MS, a proteomic tool that has emerged in recent years, has become an innovative, accurate and alternative tool for the identification of arthropods, including ticks. SIGNIFICANCE OF THE STUDY: The correct identification of ticks, including vectors responsible for the transmission of infectious diseases in humans and animals is essential for their control. This study revealed that MALDI-TOF MS is able to identify hard ticks stored during decades in alcohol or dry (evaporated alcohol). All soft ticks treated in this study by PReDO protocol exhibited low intensity spectra with background noise. The identification of ticks and their associated pathogens is important for knowledge on tick-borne diseases. Blind testing showed that 94%, and 93% of the spectra were correctly identified with relevant log score values (LSVs ≥1.8), respectively for ReDO and PReDO protocols. With new protocols named ReDO and PReDO the spectra were of good quality with high intensities (> 3000 a.u.). variegatum legs from dry (evaporated alcohol, DO-mod protocol). 93.48 of the tested ticks were correctly identified using MALDI-TOF MS technique. Knowledge about tick biodiversity in northeastern Algeria has been updated. The same observation was made on the spectra from Am. This paper assessed MALDI-TOF MS technique to identify ticks sampled on several hosts and investigated tick-borne diseases. variegatum legs preserved in alcohol for long periods with the reference protocol, named DO that we had set up years ago. This procedure represents a time-cost saving and does not require expert knowledge. Spectra of insufficient quality were obtained from Am. Recently, MALDI-TOF MS has been used as an innovative tool for the rapid and accurate identification of alcohol-preserved ticks based on proteins from extracted legs. Amblyomma (Am.) variegatum species which had been stored in alcohol for more than twenty years and for which numerous specimens were available were used to compare the performance of four protocols tested. ![]() The objective was to determine the utility of this proteomic tool for reliable species identification of closely related afrotropical ticks.Objective of this study is to find the optimal conditions for preparing the samples, resulting in quality, reproducible and specific MS spectra of the ticks, with a shelf life in 70% ethanol of more than ten years. In this study, we applied MALDI-TOF MS to a collection of laboratory and field ticks found in Eastern Africa. Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) is increasingly becoming a useful tool in arthropod identification and has the potential to overcome the limitations of classical morphology-based species identification. A simple and reproducible technique for rapid and reliable differentiation of large numbers of closely related field-collected ticks, which are often difficult and tedious to discriminate purely by morphology, will be an essential component of this strategy. Effective surveillance of tick-borne pathogens depends on accurate identification and mapping of their tick vectors. Many of these ticks are vectors of protozoan and rickettsial pathogens including Theileria parva that causes East Coast fever, a debilitating cattle disease endemic to eastern, central and southern Africa. The tick population of Africa includes several important genera belonging to the family Ixodidae. To better assess the robustness of the MALDI-TOF MS for the identification of unknown truffles, we performed a real-time blind test using a naive operator as follows.
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